Wiro Mahieu started playing the bass guitar when he was thirteen. At the age of seventeen he picked up the upright bass. He studied at the ArtEZ School of Music in Arnhem double bass under Henk Haverhoek and bassguitar under Lenee Tevoortwis, with whom he graduated in 1990. In addition, he received lessons from such guest teachers as Eddie Gomez, Miroslav Vitous and Ray Brown.

Mahieu first became known to a larger audience when he won the Soloist Prize at the 1988 NOS-Meervaart-Jazz Concours in Amsterdam, sharing this award with the Tilburg-based pianist Jeroen van Vliet. This was not to be his last award. Together with the group 'Cyprus Constructie' he received the Polaroid Jazz Award in 1990. In 1996 he won the prestigious Wessel Ilckenprijs, accompanied by pianist Rob van den Broeck, with whom he had collaborated since 1987 till 2012.

From 1989 to 1992 he went on several European tours with Luluk Purwanto and the Helsdingen Trio, featuring among others Marcello Peliterri, Donald Dean, Egbert van Gruijthuijzen and Marcel Serierse.

Between 1992 and 1997 Wiro was a part of Loek Dikker's 'Waterland Sextet' with among others Jarmo Hoogendijk, Leo van Oosterom, Cees Kranenburg Jr, John Engels and Fred Leeflang.

From 1993 to 1998 he played in the 'Vandoorn' ensemble, resulting in a cooperation with Kenny Wheeler and Thomas Chapin.

Between 1997 and 2000 Wiro played in the 'Eric Vloeimans Trio'.

In the period 1996-2005 he collaborated with the 'Rob van den Broeck International Quartet' including Tony Levin and Gerd Dudek.

In the period 1997-2006 Wiro played with the 'Jeroen Pek Quartet'.

From 1997 to 2004 Wiro performed with his own 'Low Motion Trio'. In this trio, with a central role for the upright bass, he was accompanied by Jeroen van Vliet and Pieter Bast. The 'Low Motion Trio' took the stage both in the Netherlands and abroad, including a performance at the North Sea Jazz Festival. He also made appearances in domestic and foreign jazz television shows. In 2000 the trio toured together with alto saxophonist Charlie Mariano.

Between 2000 and 2007 Wiro played in the 'Willem Hellbreker Quartet' with among others Bert van de Brink.

From 2001 to 2007 he was a part of the 'Amina Figarova Sex- en Septet' with among others Kurt van Herck and Chris Strik.

From 2002 to 2004 he toured extensively in the Netherlands and Germany with the 'Masha Bijlsma Band', featuring among others Ack van Rooyen, Tony Lakatos, Bob Mallach and Benny Bailey.

From 2004 Wiro performs with Paul van Kemenade in various line-ups. These internationally active groups collaborated with Les Freres Guisse, Bah Cissoko, David Linx, Sonti Mnebele, Feya Faku, Jeroen Zijlstra, Angelo Verploegen, Harmen Fraanje, Rik Mol, Ferhat Otay, Jeroen van Vliet, Pieter Bast, Louk Boudesteijn and Sidney Mnisi.

From 2009 until 2015 he has played in the 'Ad Colen Quartet' with Yonga Sun and Ge Bijvoet.

Ever since 1988 he works with the Colette Wickenhagen Quintet with Clous van Mechelen.

In addition, Wiro has worked with among others Michiel Borstlap, Ralph Peterson, Michael Varekamp, Dick de Graaf, Tom Beek, Guus Tangelder, Joop van Erven, Pierre Courbois, Tineke Postma, Rob van Bavel, Denise Jannah, Noa, Ernst Reijseger, Bert Joris, Benny Maupin, Colette Wickenhagen, Rinus Groeneveld, Jan Verweij, Ben Schroder, Tilman Junius, Glenn Corneille, Gijs Hendriks, Ruud Breuls, Sara Lee Vos, Marc van Vught, Jeroen Doomernik, Rembrandt Frerichs, Margriet Sjoerdsma, Nick van der Schoot, Volker Winck, Peter Weniger, Hans Peter Salentin, Eef Albers, Wim de Vries, Jaap Berends, Michael Gustorff, Maikel Thijssen, Anne Chris, Pascal Vermeer and many others.

Every year since 1988 he has made appearances on various radio and television programmes, on festivals and in jazz clubs both domestically and abroad with the above-mentioned bands and artists, visiting Germany, Belgium, France, Spain, Italy, Russia, Scandinavia, Greece, England, Ireland, South Africa, Indonesia, Switzerland, Austria and the US.